It happened on the Mission Bay Drive on-ramp to southbound Interstate 5 around 6 a.m.
Officers from both CHP and San Diego Police arrived on the scene a short time later.
Animal experts from SeaWorld San Diego responded.
Around 6:50 a.m., animal control officers managed to wrangle the pup into a vehicle so it could be transported to SeaWorld San Diego to be evaluated, San Diego police said.
No traffic delays were reported.