The ceremony will take place during the halftime show of the Chargers' Nov. 22 game at Qualcomm Stadium, to air on NBC's Sunday Night Football.
Tomlinson will be the fourth player to have his number retired by the team, joining former Chargers players Lance Alworth (19), Dan Fouts (14) and the late Junior Seau (55).
Tomlinson will be the 38th member enshrined in the Chargers' Hall of Fame.
"Few players, if any, have meant more to this franchise than LT,'' Chargers Chairman Dean Spanos said. "He was the heart and soul of this team through one of the most successful decades in our history.''
Tomlinson retired from the NFL in 2012 as the league's fifth all-time leading rusher, according to the Chargers. He is expected to be a top choice for the Pro Football Hall of Fame selection when he becomes eligible in 2017.
Tomlinson was the Chargers' first-round draft pick in the 2001 NFL draft. He was with the team for nine seasons.